prolong gold antifade  (Thermo Fisher)

Journal: bioRxiv

Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

doi: 10.64898/2025.12.04.692390

Figure Lengend Snippet: a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .

Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

Techniques:

Journal: bioRxiv

Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

doi: 10.64898/2025.12.04.692390

Figure Lengend Snippet: a, Representative immunofluorescent images of BODIPY + neutral lipids and GFAP + astrocyte in the hippocampal DG of PS19/E4 and PS19/E3 mice at 10 months of age. b-e, Correlations between BODIPY + neutral lipids in DG (% area) and Iba1 + % area ( n = 42) ( b ), AT8 + % area ( n = 31) ( c ), NeuN + % area ( n = 39) ( d ), and hippocampal volume ( n = 39) ( e ) in PS19/E3 mice, assessed via Pearson’s correlation analysis (two-sided). f, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of PS19/E4 mice at 4.0, 6.2, 7.8, and 10.0 months of age. Scale bars, 25μm. g, Correlation between age (in months) and BODIPY + neutral lipids in the hippocampal DG of PS19/E4 mice ( n = 20). Nonlinear regression R 2 . h-j, Representative immunofluorescent images of Rab5 + early endosomes (red, h ), Rab7 + late endosomes (red, i ), and LAMP1 + lysosomes (red, j ) with Iba1 + microglia (blue) and BODIPY + neutral lipid (green) in PS19/E4 mice. All scale bars, 40μm. k, Quantification of BODIPY + marker inside LAMP1 + stain ( n = 15).

Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

Techniques: Marker, Staining

Journal: bioRxiv

Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

doi: 10.64898/2025.12.04.692390

Figure Lengend Snippet: a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG mask. Quantified data in b-e represented as mean ± s.e.m., unpaired two-sided t-test. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/NSE-E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm. k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. Quantified data in k and l are represented as mean ± s.e.m., one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparisons test. For all experiments, PS19/E4, n = 35; PS19/E3 n = 42; PS19/E4/Syn1-Cre n = 29; and PS19/NSE-E4 n = 18. Pearson’s correlation analysis (two-sided) used for f-i .

Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

Techniques: